The editors of Lab Manager Magazine and Frost & Sullivan recently conducted a survey to better understand the sequencing end-user base. A total of 79 Lab Manager Magazine readers qualified for the survey as current or future users of sequencing technology, representing research and clinical laboratories, core facilities, sequencing services providers, and major genomes centers globally. This analysis investigates topics such as current and future adoption of sequencing technologies, brand and instrument preferences, outsourcing, laboratory budgets, sequencing utilization, applications, and challenges.