Global Next-generation Sequencing Trends

An End-user Perspective

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The overall research objective was to capture and analyze global next-generation (NGS) sequencing trends and use. Most researchers outsource NGS technologies; however, in-house NGS technology use is expected to increase within the next 12 months. Data accuracy and operating costs are the top criteria for purchasing sequencing instruments. Disease-related research is the dominant use for NGS technologies. The popularity of RNA-Seq continues to surge, with half of the respondents planning to use this application in the next 24 months. The most significant bottleneck in the NGS workflow is the length of time spent analyzing data. The in-house annual budget for NGS data interpretation software is more than double that of those who outsource.

Table of Contents

Research Objectives and MethodsResearch Background and ObjectivesMethodsSample DetailsExecutive SummaryExecutive SummaryExecutive Summary (continued)Outsourced Compared to In-house NGS—Current and PlannedImportance of Attributes in Purchasing Sequencing InstrumentsPrimary Research Area of NGSAverage Time Spent on Analyzing and Interpreting NGS DataAnnual Budget for Software for Analyzing and Interpreting NGS DataIn-house and Outsourcing Use Rates of Sequencing TechnologiesTechnologies Used In-house—Current and PlannedOutsourced Technologies—Current and PlannedIn-house Next-generation Sequencing DetailsAverage Number of Currently Installed/Shared NGS InstrumentsAverage Number of Currently Installed/Shared Capillary Electrophoresis Sequencing InstrumentsImportance of Attributes in Purchasing Sequencing InstrumentsTop 10 Sequencing Instruments—Installation Details by BrandsSequencing Instruments—Installation Details by BrandsPurchase Potential of New Upcoming Sequencing TechnologiesNext-generation Sequencing Outsourcing DetailsOutsourcers of NGS—Current and PlannedTop Outsourced Commercial Service Providers—Current and PlannedNext-generation Sequencing ApplicationsPrimary Research Area of NGSSequencing Applications—Current and FutureNext-generation Sequencing ReagentsNGS Reagents ProvidersOverall Experience atings of the Most sed rovidersBioinformaticsExpected Sample Throughput for NGS Projects in 2014Average Time Spent on Analyzing and Interpreting NGS DataAverage Number of Hours Spent on Analyzing and Interpreting NGS Data (continued)Annual Budget for Software for Analyzing and Interpreting NGS DataPer-sample Cost for NGS InterpretationTop Companies Used for NGS Bioinformatics PurposesTop Voted Providers of NGS Services Sequencing Data Stored in the CloudDemographic ProfileInvolvement in the Use of NGS TechnologiesLocation of the LaboratoryLocation of the OrganizationCurrent PositonLegal DisclaimerAppendix—In-house and Outsourcing Use Rates of Sequencing TechnologiesTechnologies Currently Used In-house by Lab LocationTechnologies Currently Used In-house by NGS UseTechnologies Planned to be Used In-house by Lab LocationTechnologies Planned to be Used In-house by NGS UseTechnologies Currently Outsourced by Lab LocationTechnologies Currently Outsourced by NGS UseTechnologies Planned to be Outsourced by Lab LocationTechnologies Planned to be Outsourced by NGS UseIn-house Next-generation Sequencing DetailsImportance of Attributes in Purchasing Sequencing Instruments by Lab Location Importance of Attributes in Purchasing Sequencing Instruments by Lab Location (continued) Imporance of Attributes in Purchasing Sequencing Instruments by NGS UseImporance of Attributes in Purchasing Sequencing Instruments by NGS Use (continued)Sequencing Instruments—Currently Installed/Shared by Lab LocationSequencing Instruments—Currently Installed/Shared by NGS UseSequencing Instruments—Planned to be Installed/Shared by Lab LocationSequencing Instruments—Planned to be Installed/Shared by NGS UseSequencing Instruments—No Plans to Use by Lab LocationSequencing Instruments—No Plans to Use by NGS UsePurchase Potential of New Upcoming Sequencing Technologies by Lab LocationPurchase Potential of New Upcoming Sequencing Technologies by NGS UseNext-generation Sequencing Outsourcing DetailsCurrently Used Outsourcers of NGS by Lab LocationCurrently Used Outsourcers of NGS by NGS UseNGS Outsourcers Planned to be Used by Lab LocationNGS Outsourcers Planned to be Used by NGS UseCurrently Outsourced Commercial Service ProvidersCurrently Outsourced Commercial Service Providers (continued)Commercial Service Providers Planned to be OutsourcedCommercial Service Providers Planned to be Outsourced (continued) Next-generation Sequencing ApplicationsPrimary Research Area of NGS by Lab LocationPrimary Research Area of NGS by Lab Location (continued)Primary Research Area of NGS by NGS UsePrimary Research Area of NGS by NGS Use (continued)Currently Conducted Sequencing Applications by Lab LocationCurrently Conducted Sequencing Applications by Lab Location (continued)Currently Conducted Sequencing Applications by NGS UseCurrently Conducted Sequencing Applications by NGS Use (continued)Sequencing Applications Planned to be Conducted by Lab LocationSequencing Applications Planned to be Conducted by Lab Location (continued)Sequencing Applications Planned to be Conducted by NGS UseSequencing Applications Planned to be Conducted by NGS Use (continued)Next-generation Sequencing ReagentsNGS Reagents Providers by Lab LocationNGS Reagents Providers by Lab Location (continued)NGS Reagents Providers by NGS UseNGS Reagents Providers by NGS Use (continued)BioinformaticsAverage Time Spent on Analyzing and Interpreting NGS Data by Lab LocationAverage Time Spent on Analyzing and Interpreting NGS Data by NGS UseCompanies Used for NGS Bioinformatics PurposesCompanies Used for NGS Bioinformatics Purposes (continued)Best Providers of NGS Bioinformatics Services Best Providers of NGS Bioinformatics Services (continued) Demographic ProfileInvolvement in the Use NGS Technologies by Lab LocationInvolvement in the Use NGS Technologies by NGS UseThe Frost & Sullivan StoryThe Frost & Sullivan StoryValue Proposition—Future of Your Company & CareerGlobal PerspectiveIndustry Convergence360º Research PerspectiveImplementation ExcellenceOur Blue Ocean Strategy




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